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prset b vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher prset b vector
    Prset B Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prset+b+vector/pm39190632-236-18-21
    Average 90 stars, based on 1 article reviews
    prset b vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water
    Article Snippet: To obtain Venus/cpVenus-HsCen3-Nluc, amplified PCR fragments were digested with the enzymes and inserted into the pRSET B (Invitrogen, Carlsbad, CA, USA) vector between Bam HI and Eco RI sites by four-piece ligations.

    Construct:


    Article Title: A modular enzyme with combined hemicellulose-removing and LPMO activity increases cellulose accessibility in softwood.
    Article Snippet: .. All LPMO-containing constructs, requiring periplasmic expression for correct processing of the N-terminal histidine [36,44,76], were cloned into the pRSET B vector (Invitrogen, Carlsbad, CA, USA) using the In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). ..

    Produced:


    Plasmid Preparation:


    Article Title: A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide
    Article Snippet: .. We then inserted the ELP-TEMP gene into the pRSET B vector (Invitrogen) for bacterial expression or the pcDNA3.1(-) vector (Invitrogen) for mammalian expression. ..

    Article Title: An Improved Genetically Encoded Red Fluorescent Ca 2+ Indicator for Detecting Optically Evoked Action Potentials
    Article Snippet: The cDNAs encoding R-GECO1 and ChR2 were chemically synthesized (GenScript). .. The cDNAs encoding red fluorescent GECIs were subcloned into a pRSET B vector (Invitrogen) containing a T7 promoter as described previously for bacterial expression or a pEGFP-N1 vector (Clontech) with a CMV promoter, as described previously for expression in HeLa cells and cultured rat hippocampal neurons. .. The cDNA encoding ChR2 was subcloned into a pEGFP-C1 vector (Clontech).

    Article Title: Biochemical Characterization of GacI, a Bifunctional Glycosyltransferase-Phosphatase Enzyme Involved in Acarbose Biosynthesis in Streptomyces glaucescens GLA.O.
    Article Snippet: Acarbose, a pseudotetrasaccharide produced by several strains of Actinoplanes and Streptomyces, is an α-glucosidase inhibitor clinically used to control type II diabetes.. Bioinformatic analysis of the biosynthetic gene clusters of acarbose in Actinoplanes sp. SE50/110 (the acb cluster) and Streptomyces glaucescens GLA.O (the gac cluster) revealed their distinct genetic organizations and presumably biosynthetic pathways.. However, to date, only the acarbose pathway in the SE50/110 strain has been extensively studied.

    Article Title: Genetically Encoded Green Fluorescent Ca 2+ Indicators with Improved Detectability for Neuronal Ca 2+ Signals
    Article Snippet: .. These cDNAs were subcloned into a pRSET B vector (Invitrogen) containing a T7 promoter, as described for bacterial expression, or into a pEGFP-N1 vector (Clontech) with a CMV promoter, as described for expression in HeLa cells and cultured rat hippocampal neurons. .. For in utero electroporation, cDNAs encoding G-CaMPs and mCherry (Clontech) were subcloned into a pCAGGS vector containing a CAG promoter (CMV enhancer, β-actin promoter and woodchuck hepatitis virus regulatory element [WPRE]) .

    Expressing:

    Article Title: A highly-sensitive genetically encoded temperature indicator exploiting a temperature-responsive elastin-like polypeptide
    Article Snippet: .. We then inserted the ELP-TEMP gene into the pRSET B vector (Invitrogen) for bacterial expression or the pcDNA3.1(-) vector (Invitrogen) for mammalian expression. ..

    Article Title: An Improved Genetically Encoded Red Fluorescent Ca 2+ Indicator for Detecting Optically Evoked Action Potentials
    Article Snippet: The cDNAs encoding R-GECO1 and ChR2 were chemically synthesized (GenScript). .. The cDNAs encoding red fluorescent GECIs were subcloned into a pRSET B vector (Invitrogen) containing a T7 promoter as described previously for bacterial expression or a pEGFP-N1 vector (Clontech) with a CMV promoter, as described previously for expression in HeLa cells and cultured rat hippocampal neurons. .. The cDNA encoding ChR2 was subcloned into a pEGFP-C1 vector (Clontech).

    Article Title: A modular enzyme with combined hemicellulose-removing and LPMO activity increases cellulose accessibility in softwood.
    Article Snippet: .. All LPMO-containing constructs, requiring periplasmic expression for correct processing of the N-terminal histidine [36,44,76], were cloned into the pRSET B vector (Invitrogen, Carlsbad, CA, USA) using the In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). ..

    Article Title: Genetically Encoded Green Fluorescent Ca 2+ Indicators with Improved Detectability for Neuronal Ca 2+ Signals
    Article Snippet: .. These cDNAs were subcloned into a pRSET B vector (Invitrogen) containing a T7 promoter, as described for bacterial expression, or into a pEGFP-N1 vector (Clontech) with a CMV promoter, as described for expression in HeLa cells and cultured rat hippocampal neurons. .. For in utero electroporation, cDNAs encoding G-CaMPs and mCherry (Clontech) were subcloned into a pCAGGS vector containing a CAG promoter (CMV enhancer, β-actin promoter and woodchuck hepatitis virus regulatory element [WPRE]) .

    Cell Culture:

    Article Title: An Improved Genetically Encoded Red Fluorescent Ca 2+ Indicator for Detecting Optically Evoked Action Potentials
    Article Snippet: The cDNAs encoding R-GECO1 and ChR2 were chemically synthesized (GenScript). .. The cDNAs encoding red fluorescent GECIs were subcloned into a pRSET B vector (Invitrogen) containing a T7 promoter as described previously for bacterial expression or a pEGFP-N1 vector (Clontech) with a CMV promoter, as described previously for expression in HeLa cells and cultured rat hippocampal neurons. .. The cDNA encoding ChR2 was subcloned into a pEGFP-C1 vector (Clontech).

    Article Title: Genetically Encoded Green Fluorescent Ca 2+ Indicators with Improved Detectability for Neuronal Ca 2+ Signals
    Article Snippet: .. These cDNAs were subcloned into a pRSET B vector (Invitrogen) containing a T7 promoter, as described for bacterial expression, or into a pEGFP-N1 vector (Clontech) with a CMV promoter, as described for expression in HeLa cells and cultured rat hippocampal neurons. .. For in utero electroporation, cDNAs encoding G-CaMPs and mCherry (Clontech) were subcloned into a pCAGGS vector containing a CAG promoter (CMV enhancer, β-actin promoter and woodchuck hepatitis virus regulatory element [WPRE]) .

    Clone Assay:

    Article Title: A modular enzyme with combined hemicellulose-removing and LPMO activity increases cellulose accessibility in softwood.
    Article Snippet: .. All LPMO-containing constructs, requiring periplasmic expression for correct processing of the N-terminal histidine [36,44,76], were cloned into the pRSET B vector (Invitrogen, Carlsbad, CA, USA) using the In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). ..

    Cloning:

    Article Title: A modular enzyme with combined hemicellulose-removing and LPMO activity increases cellulose accessibility in softwood.
    Article Snippet: .. All LPMO-containing constructs, requiring periplasmic expression for correct processing of the N-terminal histidine [36,44,76], were cloned into the pRSET B vector (Invitrogen, Carlsbad, CA, USA) using the In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). ..



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